Prevalence and characterization of extended spectrum beta-lactamase-producing Enterobacter cloacae strains in Algeria.
Identifieur interne : 000215 ( Main/Exploration ); précédent : 000214; suivant : 000216Prevalence and characterization of extended spectrum beta-lactamase-producing Enterobacter cloacae strains in Algeria.
Auteurs : Sabrina Nedjai [Algérie] ; Abouddihaj Barguigua ; Nassima Djahmi ; Loubna Jamali ; Khalid Zerouali ; Mazouz Dekhil ; Mohammed TiminouniSource :
- Journal of infection in developing countries [ 1972-2680 ] ; 2013.
Descripteurs français
- KwdFr :
- Adolescent (MeSH), Adulte (MeSH), Adulte d'âge moyen (MeSH), Algérie (épidémiologie), Analyse de regroupements (MeSH), Enfant (MeSH), Enfant d'âge préscolaire (MeSH), Enterobacter cloacae (classification), Enterobacter cloacae (enzymologie), Enterobacter cloacae (génétique), Enterobacter cloacae (isolement et purification), Femelle (MeSH), Génotype (MeSH), Humains (MeSH), Hôpitaux (MeSH), Infections à Enterobacteriaceae (microbiologie), Infections à Enterobacteriaceae (épidémiologie), Jeune adulte (MeSH), Mâle (MeSH), Nourrisson (MeSH), Nouveau-né (MeSH), Prévalence (MeSH), Réaction de polymérisation en chaîne (MeSH), Sujet âgé (MeSH), Tests de sensibilité microbienne (MeSH), Typage moléculaire (MeSH), bêta-Lactamases (génétique), bêta-Lactamases (métabolisme), Électrophorèse en champ pulsé (MeSH), Épidémiologie moléculaire (MeSH).
- MESH :
- enzymologie : Enterobacter cloacae.
- génétique : Enterobacter cloacae, bêta-Lactamases.
- isolement et purification : Enterobacter cloacae.
- microbiologie : Infections à Enterobacteriaceae.
- métabolisme : bêta-Lactamases.
- épidémiologie : Algérie, Infections à Enterobacteriaceae.
- Adolescent, Adulte, Adulte d'âge moyen, Analyse de regroupements, Enfant, Enfant d'âge préscolaire, Femelle, Génotype, Humains, Hôpitaux, Jeune adulte, Mâle, Nourrisson, Nouveau-né, Prévalence, Réaction de polymérisation en chaîne, Sujet âgé, Tests de sensibilité microbienne, Typage moléculaire, Électrophorèse en champ pulsé, Épidémiologie moléculaire.
English descriptors
- KwdEn :
- Adolescent (MeSH), Adult (MeSH), Aged (MeSH), Algeria (epidemiology), Child (MeSH), Child, Preschool (MeSH), Cluster Analysis (MeSH), Electrophoresis, Gel, Pulsed-Field (MeSH), Enterobacter cloacae (classification), Enterobacter cloacae (enzymology), Enterobacter cloacae (genetics), Enterobacter cloacae (isolation & purification), Enterobacteriaceae Infections (epidemiology), Enterobacteriaceae Infections (microbiology), Female (MeSH), Genotype (MeSH), Hospitals (MeSH), Humans (MeSH), Infant (MeSH), Infant, Newborn (MeSH), Male (MeSH), Microbial Sensitivity Tests (MeSH), Middle Aged (MeSH), Molecular Epidemiology (MeSH), Molecular Typing (MeSH), Polymerase Chain Reaction (MeSH), Prevalence (MeSH), Young Adult (MeSH), beta-Lactamases (genetics), beta-Lactamases (metabolism).
- MESH :
- chemical , genetics : beta-Lactamases.
- classification : Enterobacter cloacae.
- enzymology : Enterobacter cloacae.
- epidemiology : Algeria, Enterobacteriaceae Infections.
- genetics : Enterobacter cloacae.
- isolation & purification : Enterobacter cloacae.
- chemical , metabolism : beta-Lactamases.
- microbiology : Enterobacteriaceae Infections.
- Adolescent, Adult, Aged, Child, Child, Preschool, Cluster Analysis, Electrophoresis, Gel, Pulsed-Field, Female, Genotype, Hospitals, Humans, Infant, Infant, Newborn, Male, Microbial Sensitivity Tests, Middle Aged, Molecular Epidemiology, Molecular Typing, Polymerase Chain Reaction, Prevalence, Young Adult.
Abstract
INTRODUCTION
Expended spectrum β-lactamase (ESBL)-producing Enterobacter cloacae is an important nosocomial pathogen. In this study, the prevalence and the molecular epidemiology of ESBL producing E. cloacae strains isolated from various hospitals in Annaba, Algeria were investigated.
METHODOLOGY
The study involved 63 isolates of E. cloacae obtained during 2009 at the four hospitals in Annaba. The detection of ESBL was performed using the double-disk synergy test and the combined disk test. Minimum inhibitory concentrations (MICs) were determined using the agar dilution method. The presence of bla(CTX-M), bla(SHV), bla(TEM), and bla(DHA) β-lactamase genes was evaluated by PCR, and genomic typing was determined by pulsed-field gel electrophoresis (PFGE) analysis. The clinical and microbiological data were entered into the EpiI Info database.
RESULTS
Thirty isolates (47.6%) had an ESBL phenotype. Bla(CTX-M) group1 (76%); bla(TEM) (70%) were the most prevalent, followed by bla(DHA) (16.6%) and bla(SHV) (10%). Eighteen strains expressed at least two bla genes. MICs revealed a high level of resistance to cefotaxime, ceftazidime, and cefepime. PFGE revealed an epidemic clonal dissemination of these isolates. Various risk factors associated with the occurrence of ESBL-producing E. cloacae were detected.
CONCLUSIONS
A higher frequency of ESBL-producing isolates and a diversity of β-lactamases were detected among ESBL-producing E. cloacae; these resulted from an epidemic clonal dissemination and high transference of ESBL genes between bacteria in hospital settings. Strict measures will be required to control the further spread of these pathogens in hospital settings.
DOI: 10.3855/jidc.3127
PubMed: 24240037
Affiliations:
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Le document en format XML
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adolescent (MeSH)</term>
<term>Adult (MeSH)</term>
<term>Aged (MeSH)</term>
<term>Algeria (epidemiology)</term>
<term>Child (MeSH)</term>
<term>Child, Preschool (MeSH)</term>
<term>Cluster Analysis (MeSH)</term>
<term>Electrophoresis, Gel, Pulsed-Field (MeSH)</term>
<term>Enterobacter cloacae (classification)</term>
<term>Enterobacter cloacae (enzymology)</term>
<term>Enterobacter cloacae (genetics)</term>
<term>Enterobacter cloacae (isolation & purification)</term>
<term>Enterobacteriaceae Infections (epidemiology)</term>
<term>Enterobacteriaceae Infections (microbiology)</term>
<term>Female (MeSH)</term>
<term>Genotype (MeSH)</term>
<term>Hospitals (MeSH)</term>
<term>Humans (MeSH)</term>
<term>Infant (MeSH)</term>
<term>Infant, Newborn (MeSH)</term>
<term>Male (MeSH)</term>
<term>Microbial Sensitivity Tests (MeSH)</term>
<term>Middle Aged (MeSH)</term>
<term>Molecular Epidemiology (MeSH)</term>
<term>Molecular Typing (MeSH)</term>
<term>Polymerase Chain Reaction (MeSH)</term>
<term>Prevalence (MeSH)</term>
<term>Young Adult (MeSH)</term>
<term>beta-Lactamases (genetics)</term>
<term>beta-Lactamases (metabolism)</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>Adolescent (MeSH)</term>
<term>Adulte (MeSH)</term>
<term>Adulte d'âge moyen (MeSH)</term>
<term>Algérie (épidémiologie)</term>
<term>Analyse de regroupements (MeSH)</term>
<term>Enfant (MeSH)</term>
<term>Enfant d'âge préscolaire (MeSH)</term>
<term>Enterobacter cloacae (classification)</term>
<term>Enterobacter cloacae (enzymologie)</term>
<term>Enterobacter cloacae (génétique)</term>
<term>Enterobacter cloacae (isolement et purification)</term>
<term>Femelle (MeSH)</term>
<term>Génotype (MeSH)</term>
<term>Humains (MeSH)</term>
<term>Hôpitaux (MeSH)</term>
<term>Infections à Enterobacteriaceae (microbiologie)</term>
<term>Infections à Enterobacteriaceae (épidémiologie)</term>
<term>Jeune adulte (MeSH)</term>
<term>Mâle (MeSH)</term>
<term>Nourrisson (MeSH)</term>
<term>Nouveau-né (MeSH)</term>
<term>Prévalence (MeSH)</term>
<term>Réaction de polymérisation en chaîne (MeSH)</term>
<term>Sujet âgé (MeSH)</term>
<term>Tests de sensibilité microbienne (MeSH)</term>
<term>Typage moléculaire (MeSH)</term>
<term>bêta-Lactamases (génétique)</term>
<term>bêta-Lactamases (métabolisme)</term>
<term>Électrophorèse en champ pulsé (MeSH)</term>
<term>Épidémiologie moléculaire (MeSH)</term>
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<keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Enterobacter cloacae</term>
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<keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Enterobacter cloacae</term>
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<term>Enterobacteriaceae Infections</term>
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<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Enterobacter cloacae</term>
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<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Enterobacter cloacae</term>
<term>bêta-Lactamases</term>
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<keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Enterobacter cloacae</term>
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<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Enterobacter cloacae</term>
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<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>beta-Lactamases</term>
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<keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Infections à Enterobacteriaceae</term>
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<keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Enterobacteriaceae Infections</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>bêta-Lactamases</term>
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<keywords scheme="MESH" qualifier="épidémiologie" xml:lang="fr"><term>Algérie</term>
<term>Infections à Enterobacteriaceae</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Adolescent</term>
<term>Adult</term>
<term>Aged</term>
<term>Child</term>
<term>Child, Preschool</term>
<term>Cluster Analysis</term>
<term>Electrophoresis, Gel, Pulsed-Field</term>
<term>Female</term>
<term>Genotype</term>
<term>Hospitals</term>
<term>Humans</term>
<term>Infant</term>
<term>Infant, Newborn</term>
<term>Male</term>
<term>Microbial Sensitivity Tests</term>
<term>Middle Aged</term>
<term>Molecular Epidemiology</term>
<term>Molecular Typing</term>
<term>Polymerase Chain Reaction</term>
<term>Prevalence</term>
<term>Young Adult</term>
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<keywords scheme="MESH" xml:lang="fr"><term>Adolescent</term>
<term>Adulte</term>
<term>Adulte d'âge moyen</term>
<term>Analyse de regroupements</term>
<term>Enfant</term>
<term>Enfant d'âge préscolaire</term>
<term>Femelle</term>
<term>Génotype</term>
<term>Humains</term>
<term>Hôpitaux</term>
<term>Jeune adulte</term>
<term>Mâle</term>
<term>Nourrisson</term>
<term>Nouveau-né</term>
<term>Prévalence</term>
<term>Réaction de polymérisation en chaîne</term>
<term>Sujet âgé</term>
<term>Tests de sensibilité microbienne</term>
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<front><div type="abstract" xml:lang="en"><p><b>INTRODUCTION</b>
</p>
<p>Expended spectrum β-lactamase (ESBL)-producing Enterobacter cloacae is an important nosocomial pathogen. In this study, the prevalence and the molecular epidemiology of ESBL producing E. cloacae strains isolated from various hospitals in Annaba, Algeria were investigated.</p>
</div>
<div type="abstract" xml:lang="en"><p><b>METHODOLOGY</b>
</p>
<p>The study involved 63 isolates of E. cloacae obtained during 2009 at the four hospitals in Annaba. The detection of ESBL was performed using the double-disk synergy test and the combined disk test. Minimum inhibitory concentrations (MICs) were determined using the agar dilution method. The presence of bla(CTX-M), bla(SHV), bla(TEM), and bla(DHA) β-lactamase genes was evaluated by PCR, and genomic typing was determined by pulsed-field gel electrophoresis (PFGE) analysis. The clinical and microbiological data were entered into the EpiI Info database.</p>
</div>
<div type="abstract" xml:lang="en"><p><b>RESULTS</b>
</p>
<p>Thirty isolates (47.6%) had an ESBL phenotype. Bla(CTX-M) group1 (76%); bla(TEM) (70%) were the most prevalent, followed by bla(DHA) (16.6%) and bla(SHV) (10%). Eighteen strains expressed at least two bla genes. MICs revealed a high level of resistance to cefotaxime, ceftazidime, and cefepime. PFGE revealed an epidemic clonal dissemination of these isolates. Various risk factors associated with the occurrence of ESBL-producing E. cloacae were detected.</p>
</div>
<div type="abstract" xml:lang="en"><p><b>CONCLUSIONS</b>
</p>
<p>A higher frequency of ESBL-producing isolates and a diversity of β-lactamases were detected among ESBL-producing E. cloacae; these resulted from an epidemic clonal dissemination and high transference of ESBL genes between bacteria in hospital settings. Strict measures will be required to control the further spread of these pathogens in hospital settings.</p>
</div>
</front>
</TEI>
<affiliations><list><country><li>Algérie</li>
</country>
</list>
<tree><noCountry><name sortKey="Barguigua, Abouddihaj" sort="Barguigua, Abouddihaj" uniqKey="Barguigua A" first="Abouddihaj" last="Barguigua">Abouddihaj Barguigua</name>
<name sortKey="Dekhil, Mazouz" sort="Dekhil, Mazouz" uniqKey="Dekhil M" first="Mazouz" last="Dekhil">Mazouz Dekhil</name>
<name sortKey="Djahmi, Nassima" sort="Djahmi, Nassima" uniqKey="Djahmi N" first="Nassima" last="Djahmi">Nassima Djahmi</name>
<name sortKey="Jamali, Loubna" sort="Jamali, Loubna" uniqKey="Jamali L" first="Loubna" last="Jamali">Loubna Jamali</name>
<name sortKey="Timinouni, Mohammed" sort="Timinouni, Mohammed" uniqKey="Timinouni M" first="Mohammed" last="Timinouni">Mohammed Timinouni</name>
<name sortKey="Zerouali, Khalid" sort="Zerouali, Khalid" uniqKey="Zerouali K" first="Khalid" last="Zerouali">Khalid Zerouali</name>
</noCountry>
<country name="Algérie"><noRegion><name sortKey="Nedjai, Sabrina" sort="Nedjai, Sabrina" uniqKey="Nedjai S" first="Sabrina" last="Nedjai">Sabrina Nedjai</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>
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